Importing Ingredients Using the DoE Tool


Important: The DoE tool cannot be used to assign pH to salt(s).

ROCK MAKER enables you to quickly create grid layers in your experiments without manually defining the conditions for each well, by using the DoE tool. When you use the DoE, you will be asked to complete the following tasks:

.CSV Template File for the DoE Tool

Important: There should be a pH column for every buffer in your file, as shown in the image above.

In this section, you will learn how to import ingredients using the DoE feature in ROCK MAKER.




To import ingredients using the DoE:

  1. Create a new experiment, or open an existing experiment.
  2. Add a grid layer to the canvas. This grid layer should contain at least as many wells as in the .CSV file.
  3. Click Add Ingredient.

Add Ingredients in the Edit Grid Layer Panel

  1. Click anywhere in the ingredient Name box to display the Select Ingredients panel.

  2. Type or select an ingredient and its relevant stock.

Note: Make sure that the stock you select for an ingredient is at a higher concentration than the highest concentration for that ingredient in your .CSV file. Similarly, the stocks for a certain buffer should be selected such that they cover the range of pH for that buffer in the file. For example, if your .CSV contains TRIS within a concentration range of 0.05-0.20 M, and a pH range of 7-9, the stocks should be 1 M TRIS pH =< 7 and 1 M TRIS pH >= 9.

Type or Select an Ingredient


  1. Under Concentration and pH (buffers only), select Vary Custom. Then, click Add.

Select Vary Custom for the Concentration and pH


  1. Repeat steps 3 - 6 to add additional ingredients.

A Grid Layer with Ingredients in the Canvas


  1. When you have finished adding your ingredients, click the DoE button on the Edit Grid Layer panel.
  2. Locate the .CSV file that you want to import and click Open.

Selecting a .CSV File to Import

  1. After the Import DoE window appears, you will need to match the imported data with the ingredients you just added in ROCK MAKER. The Import DoE window consists of two main panels, as follows:


    Important: You cannot match pH data to a salt.

    The Import DoE Window


  2. In the Match to column of the Match Imported Data panel, select ingredients from the list accordingly. For example, the name of the ingredient in the first row of your .CSV file may be written “Tris Conc (mM)”, but the actual name of the ingredient in your RM database is “TRIS”. So, for each item in the Imported Data column, you must specify the relevant Ingredient in the Match to column. During the matching process, the unit for pH data should must be selected as pH.

Match Your Ingredients in the Import DoE Window

  1. When you are done, click Import.
  2. Your imported ingredients will automatically be generated in the grid layer that you previously created. You are now ready to continue working on your experiment.

The Imported Ingredients have been Generated in the Grid Layer

Note: If your grid layer contains more wells than the conditions in the .CSV file, the extra wells will be filled with water. If your grid layer contains fewer wells than the conditions in the .CSV file, the extra conditions will be ignored.


Related Topics


10 DeAngelo Drive

Bedford, MA 01730