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The LCP option includes an LCP mixer, used for combining the monoolein with the protein sample. This tutorial explains how to set up mixing in the software and hardware.
Important: The maximum total volume of the two syringes to be mixed should be 70 μL.
Click 
. The Mixing LCP Wizard dialog displays (see below).
Mixing LCP Wizard
Number of mixing cycles: Sets number of mixing cycles to be performed by the NT8.
Mixing speed: Sets the mixing speed to be performed by the NT8.
Specify the settings on the LCP Mixing Wizard as needed.
Click OK to confirm. A notification appears and asks you to connect the mixer plunger to the stage.
Connect the mixer plunger to the stage.
The mixer plunger should be positioned on the top of the reference hole located on the stage.
Bring the mixer plunger to the hole by sliding it.
The Mixer Plunger
Press down the locking knob until it gets into the hole and tighten the knob by turning it counterclockwise. Please make sure it firmly locks the mixer plunger.
Tightening the Knob
Prepare two LCP glass syringes that have been filled with solution and protein.
Mount the coupler on the LCP syringe, and join the two LCP syringes using the coupler. Make sure both syringes are conjoined.
Joining the two LCP syringes using the coupler
Install the syringe on the mixer by inserting it into the mixer slots. When you insert the syringe, make sure it reaches the stopper.
The Syringe on the Mixer
After you are done setting up the NT8 software and hardware, you are now ready to mix the LCP drops. To mix the LCP drops, follow these steps:
In the software, click OK to confirm that you have connected the syringe to the mixer station. The system mixes the syringe.
After the mixing is finished, a new dialog displays and notifies you to remove the LCP syringe from the stage.
Push the plunger to the other syringe position. Detach the conjoined syringes. Attach the needle to the syringe that will be used in your experiment.
Disconnect the mixer plunger from the stage: Push down on the locking knob and turn it clockwise.
Click OK.
Mixing LCP dialog box
Click Finish. You can now use the mixed protein and solution.
Mixing LCP Wizard
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NTC-V28R016
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