You are here: Administrative Tasks : Sequence Editor : Setting Up and Managing Sequence Editor

Setting Up and Managing Sequence Editor

Note: It is recommended that the Sequence Editor be used only by advanced MANTIS users.

Follow the instructions below to add a new sequence and modify the existing sequence in the Sequence Editor window

Adding a New Sequence

To create a new sequence:

In the MANTIS software:
- Go to the Tools menu and select Sequence Editor.
  
  OR
- Press Ctrl+Shift+O on your keyboard.
Opening the Sequence Editor
OPTIONAL: Click the Create new Sequence button to clear the previous sequence from the Sequence Item Table.
Add the sequence items based on your needs:
Valve
- Select from the following options:
1. Click the Add Item button, and select Valve . The valve state will automatically appear in the Sequence Item Table with the latest configuration. Then, change the configuration by opening (green) and closing (red) the valves by clicking on the desired area in the Valve Control Panel. Click Write State apply the GUI valve state to the selected Valve sequence item. From the Valve Control Panel, manually open (green) and close (red) valves by clicking on the desired area. Click the Add Item button and select Valve. The graphical valve state will be transferred over to the Sequence Item Table.
Move
1. Select Move from the Add Item button .
2. In the Move Sequence Item window:
  1. Set the Movement Type by choosing from the following options:
    
    Absolute — to move the MANTIS hand to the determined location based on the X, Y, and Z axis coordinates.
    
    Relative — to move the MANTIS hand along the selected axes by a given amount, defined by the change of each value (dX, dY, and dZ).
    
    Selecting Movement Type
  2. OPTIONAL: Click Copy Position to duplicate the current coordinate to each axis field in the Active Axis section.
  3. Configure the X/dX, Y/dY, and Z/dZ values from the Active Axis section by selecting and setting the preferred axis coordinates.
  4. Click OK to save the changes and close the Move Sequence Item window.
Label
1. Select Label from the Add Item button .
2. In the Label Sequence Item window, enter the label name.
  
  Typing Label Name
3. Click OK to save the label in the Sequence Item Table.
Go To
1. Select Go To from the Add Item button .
2. In the Go To Sequence Item window, select the available label that has been assigned to the Sequence Item Table.
  
  Selecting the Label
3. Click OK.
Air Pump
1. Select Air Pump from the Add Item button .
2. In the Air Pump Sequence Item window:
  1. Set the delay time (in seconds) on the Timer field.
  2. Turn the air pump on/off by selecting from the available options.
    
    Air Pump Sequence Item Window
    
    Note: When turning the pump off, MANTIS will pause for the specified time before turning off the air pump. When turning the pump on, MANTIS will pause for the specified time after turning on the pump and before moving to the next command.
3. Click OK to save the configuration.
Plate Clamp
1. Select the Plate Clamp option from the Add Item button .
2. Set the plate clamp status to open/close by selecting Lock Plate Clamp (close) or Unlock Plate Clamp (open).
3. Click OK to save the settings.
  
  Plate Clamp Sequence Item Window
Timed Valve
1. Select Timed Valve from the Add Item button .
2. In the Timer Sequence Item window:
  1. Select the Open then Close option to close and open the valve accordingly, or Close then Open to open and then close the valve.
  2. Set the delay period (in milliseconds) in the Delay (ms) field that MANTIS will hold the valves in their initial state.
  3. OPTIONAL: Select the Edit Toggle Valve option to edit the output, input, and common valve (plate clamp, pressure/vacuum, and air pump) manually.
    
    Timer Sequence Item Window
3. Click OK to save the changes.
Pressure Control
1. Select Pressure Control from the Add Item button .
2. In the Pressure Regulator Sequence Item window:
  1. Select the Pressure Type from the available selections.
  2. Set the period (in milliseconds) for MANTIS to wait for the target pressure/vacuum value.
  3. Set the target pressure (in psi) you want MANTIS to achieve in the Target Pressure field.
  4. OPTIONAL: Select the Ignore Warning option to hide the warning message that informs you if the target pressure is failed to achieve during the delay period. By ignoring the message, MANTIS will automatically use the current pressure value, which may cause inaccurate dispense result.
    
    Pressure Regulator Sequence Item Window
3. Click OK when you’re done.
Carousel*
1. Select Carousel from the Add Item button .
2. Select the left/right position for LC3 on MANTIS.
3. Set the chip position of the carousel to determine the L1/R1 location from the Index field.
4. Click OK when you’re done.
  
  Carousel Sequence Item Window
LC3*
1. Select LC3 from the Add Item button .
2. Select the available LC3 serial number from the LC3 S/N field.
3. Configure the rotation degree of the carousel from the Degree field.
4. OPTIONAL: Click Copy Position to duplicate the current degree position of the carousel rotation angle.
5. Click OK when you’re done.
  
  LC3 Sequence Item Window
*Available if MANTIS is connected to the LC3.
Choose from the following options:
- Save the custom sequence:
  1. Click the Save Sequence button .
  2. In the Specify Sequence File window, enter the sequence name in the File name field.
  3. Click Save. The sequence file will be automatically saved in the User folder in .seq.txt format.
- Run the sequence by clicking the Run Sequence button . You can pause during the sequence execution or stop the sequence by clicking the Stop button.

Modifying an Existing Sequence

Occasionally, you might want to modify the sequence that you have created previously. For example, if you want to run a lifetime testing on MANTIS. Follow the steps accordingly to modify the existing sequence.

Important: Modifying default sequences in the database may affect the configurations in the MANTIS system. Please contact FORMULATRIX support for further questions or assistance.

Prerequisites:

You have created a custom sequence in your MANTIS database.

To modify the existing sequence:

Choose from the following options:
- To directly modify the custom sequence:
  1. Open the Sequence Editor window from the Tools menu in the MANTIS software.
  2. Open your custom sequence by clicking the Load Sequence button .
  3. Locate the sequence file of your preference in the User folder or your local directory. Double-click on the selected file or click Open.
    
    Selecting the Sequence File
- To modify the sequence of the existing dispense list:
  1. Load a dispense list and assign the reagent to the Input Control Panel.
  2. Open the Sequence Editor window from the Tools menu.
Choose from the following options to modify your sequence.
- To add more sequence items: click on a command in the Sequence Editor Table, then follow step 3 in creating the new sequence.
- To remove a sequence item from the Sequence Editor Table, select a command you want to remove, then click Delete Item .
- To modify the existing sequence item:
  1. Double-click on the VS, M, TVS, PR, CS or LC3 line.
  2. Then, edit the values as needed.
  3. Click OK to save the changes.
- To edit the delay time, double-click on the delay column of the selected line, input the delay time, and then press Enter on your keyboard to save the changes.
- Use the drag and drop operation to rearrange the order of the sequences.
OPTIONAL: Run the modified sequence by clicking the Run Sequence button . You can pause during the sequence execution or stop the sequence by clicking the Stop button .

Note: During the sequence execution, the blue highlight will move accordingly based on the stage of the commands in the Sequence Editor Table. You can disable this display by setting the Update Sequence Editor During Dispense option to False. For more information, read the Dispense Settings section in the Using the Options Menu topic.

Update Sequence Editor During Dispense Option
OPTIONAL: Save your modified sequence by clicking the Save button , and then select the directory in the User folder or the destination of your preference. MANTIS will not overwrite the configuration of the previous sequence, so it can be used for the next experiment in the future.