The UV light path is best explained with a diagram.
The excitation light is projected onto the protein crystal via the condenser lens - dichroic - UV objective optical chain. (Blue arrow)
The tryptophan amino acid flouresces under the UV illumination. (Red arrow)
The emission light passes through the UV objective, dichroic, emission filter, and is focused on the CCD sensor by a UV relay lens.
Key: Blue lines indicate excitation light, red lines indicate emission light.
The Diagram of How UV Imaging Works
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