Creating a Protein Layer

Each protein layer specifies the protein formulation to be used in one drop of each well in the layer. For example, if your plate has two drops per well, and your design uses both drops, then you will need to create two protein layers.

Prerequisite:

• You already have a protein formulation. Go to Creating a Protein Formulation for more information.

To create a protein layer:

1. Open an experiment OR create a new one.

2. On the experiment page, go to the Canvas menu. Then, click Add Layer on the top bar and select Protein Layer.

   Note

   You can add a protein layer automatically by adding a protein from the Explore tab in the side panel.

   

   Selecting Protein Layer

3. Select a protein layer area accordingly.

   1. To select all wells, click any well on the canvas.

   2. To select some wells, click and hold the left button of your mouse while dragging it over some desired wells.

4. Select a protein from the Select Protein side panel. By default, ROCK MAKER will show all proteins from your ROCK MAKER database.

   Then, select a protein by:

   • Typing a protein name in the Search here field for a quick search.

     OR

   • Clicking one of the available proteins.

     OR

   • Clicking the Folder button on the top right to select a protein from a different folder.

     

     Selecting a Protein

5. After protein selection, adjust the protein settings in the Edit Protein panel.

   

   Adjusting Protein Settings

   • Click the Name field to change the protein.

   • Select a protein volume variation from the Vary Along field.

   • Type a start protein volume in the Start field.

   • Select a well solution volume variation from the Vary Along field.

   • Type a start well solution volume in the Start field.

   Then, click Apply.

6. In the Protein subtab, you can modify a protein setting by clicking the Edit and Delete buttons.

   

   Protein Subtab

7. Go to the Layers subtab in the Layers Design tab and do the following:

   • Type a name in the Layer Name field or use the default name.

   • Select a target, Drop 1 or Drop 2 from the Target dropdown menu.

   • If necessary, lock the layer by clicking the Locked checkbox.

   

   Layers Subtab

8. Click Save on the top right toolbar.