Creating a Fragment Screen

Prerequisite:

• You have created a project to store fragment screens.

To create a fragment screen:

1. Open a project.

2. Locate the Administrative Tools section, click the NEW button, and select Fragment Screen. The created Fragment Screen automatically opens in a new tab.

   

   Selecting Fragment Screen

3. ROCK MAKER automatically names the created fragment screen New Fragment Screen, New Fragment Screen 2, New Fragment Screen 3, etc., following the creation sequence. You can change the name by entering the intended name in the Fragment Screen Name field of the Fragment menu. Then, click Save on the top right bar.

   Note

   The name must be unique.

   

   Fragment Screen Window

Creating a Fragment Screen Condition

Upon a fragment screen creation, you can define its conditions.

To create a fragment screen condition:

1. Open a fragment screen.

2. In the Fragment menu, locate the Add Condition field and define the number of conditions. Then, click the Add button .

   The number of conditions you define determines the number of rows created.

   

   Defining the Number of Conditions

   Then, enter:

   • The fragment name in the Fragment Name field.

   • The fragment size in the Size field.

     Important

     The value can’t be zero (0).

   • The fragment concentration in the Conc. (mM) field.

   • OPTIONAL: Any additional information for each fragment in the Notes field. For example, you can mention that a certain fragment is used in a separate successful research on a different protein.

   Note

   ROCK MAKER automatically generates the Cond. Num and ID fields.

3. If everything is set, click Save on the top right bar.

You can also create a fragment screen condition by importing a fragment condition CSV file.

To create a fragment screen condition by importing a fragment condition CSV file:

1. Open a fragment screen.

2. On the top right bar, click the Import button .

   

   Import Button

3. Select a fragment condition CSV file.

   Important

   Click Template on the top right to download the CSV template, or you can create your own CSV containing the following columns: Cond. Num, ID, Fragment Name, Size, Conc. (mM), and Notes (optional).

4. If everything is set, click Save on the top right bar.

Modifying a Fragment Screen

Renaming a Fragment Screen

Important

You can’t rename a fragment screen used in an experiment.

To rename a fragment screen:

1. Open a fragment screen.

2. In the Fragment menu, click the Fragment Screen Name field and enter the new name.

   Note

   The name must be unique.

   

   Fragment Screen Name Field

3. Click Save on the top right bar.

Copying a Fragment Screen

To copy a fragment screen:

1. Open a project.

2. Select a fragment screen(s) you want to copy and click the Copy button .

   Note

   To copy multiple fragment screens, hold Ctrl while clicking on the fragment screens you want to copy.

3. Paste the fragment screen to the same or different project.

   

   Copying and Pasting a Fragment Screen to Another Project

   Note

   If you paste the fragment screen to the same project, the name of the copied version begins with Copy and is followed by the original name. See the example below.

   

   Copying and Pasting a Fragment Screen to the Same Project

Deleting a Fragment Screen

Important

A fragment screen currently used in an experiment cannot be deleted.

To delete a fragment screen:

1. Open a project.

2. Select a fragment screen(s) you want to delete and click the Delete button .

   To delete multiple fragment screens, hold Ctrl while clicking on the fragment screens you want to delete.

   Note

   In multiple deletions, you cannot delete all fragment screens you select if one of them is used in an experiment. Unselect the one used in an experiment to delete the rest.

3. In the Confirmation dialog box, click YES, PROCEED.

   

   Confirmation Dialog Box

Modifying a Fragment Screen Condition

Adding a Fragment Screen Condition to an Existing Fragment Screen

Important

You can’t add a fragment screen condition if the fragment screen is used in an experiment.

To add a fragment screen condition to an existing fragment screen:

1. Go to a project and open a fragment screen.

2. In the Fragment submenu, click the Add button to add a new row.

   Then, enter:

   • The fragment name in the Fragment Name field.

   • The fragment size in the Size field.

     Important

     The value can’t be zero (0).

   • The fragment concentration in the Conc. (mM) field.

   • OPTIONAL: Any additional information for each fragment in the Notes field. For example, you can mention that a certain fragment is used in a separate successful research on a different protein.

   Note

   ROCK MAKER automatically generates the Cond. Num and ID fields.

   

   A New Row of Fragment Screen Condition

3. If everything is set, click Save on the top right bar.

Editing a Fragment Screen Condition

Tip

To select all fragment screen conditions, click the top checkbox in the table.

Top Checkbox

Important

You can’t edit a fragment screen condition if the fragment screen is used in an experiment.

To edit a fragment screen condition:

1. Go to a project and open a fragment screen.

2. In the Fragment submenu, select a fragment screen condition and edit the desired field(s), such as Fragment Name, Size, Conc. (mM), and/or Notes.

3. If everything is set, click Save on the top right bar.

Deleting a Fragment Screen Condition

To delete a fragment screen condition:

1. Open a project and select a fragment screen.

2. In the Fragment submenu, select a fragment screen condition(s). This activates the Delete button on the top right bar.

3. Click the Delete button.

   Important

   You cannot delete all fragment screen conditions you select if one of them is used in an experiment. Unselect the one used in an experiment to delete the rest.

   

   Delete Button

4. If everything is set, click Save on the top right bar.