Creating a Protein Variant

A protein variant is a mix of a protein formulation and other ingredients. Although derived from a protein formulation, a protein variant can be applied independently in an experiment by using the Protein Layer option.

Create Protein Variant Dialog Box

Prerequisite:

• You have a protein formulation.

To create a protein variant:

1. Go to the Explore menu and click a protein formulation. The protein formulation you choose is the parent of the protein variant you create.

2. Click the CREATE VARIANT button in the right panel to open the Create Protein Variant dialog box.

   

   Create Variant Button

   The dialog box consists of Selected Protein Details, displaying the protein formulation you chose, and Variable Protein sections.

   

   Create Protein Variant Dialog Box

3. In the Variable Protein section of the dialog box, add ingredients by manually adding them or importing a protein file.

   

   Variable Protein Section

   Adding Ingredients Manually

   To add ingredients manually:

   1. Click at the top right of the Ingredient section.

   2. Complete all columns in the new ingredient row.

      • Select an ingredient type in the Type column.

      • Enter

        • the ingredient name in the Name column.

        • the ingredient ID in the ID column. It helps assign an ingredient tracking number if you use a database other than ROCK MAKER.

        • the lot number in the Lot column. It helps track an ingredient lot number stored in a database other than ROCK MAKER.

        • the molecular weight of the ingredient in kilodaltons in the MW(kDa) column.

        • the ingredient concentration in milligrams per milliliter in the Conc. (mg/mL) column. This automatically generates the value in the Conc. (mM) column.

          

          Adding Ingredients

   3. If everything is set, click the Save button on the top right bar.

   Adding Ingredients by Importing a TXT Protein File

   Prerequisite:

   • You have a TXT protein file. Otherwise, download the template by clicking the Download Protein Template link.

     

     Download Protein Template Link

   To import a protein file:

   1. Click the IMPORT button at the top right of the Ingredient section.

      

      Import Button

   2. In the dialog box, select a TXT protein file and click OPEN. Then, the data from the TXT file is added to the Ingredient section.

      

      Selecting a TXT file

   3. If everything is set, click the Save button on the top right bar.

   Note

   You can add ingredients manually or import a protein file. If the protein file contains items identical to the manually added one, data from both will be kept. However, an error will appear if you try to save it.

Related Topics:

• Protein Formulation

• Creating a Protein Formulation

• Ingredients Setup Submenu

• Creating a Protein Type