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Creating and Using Protocols
The MANTIS software enables you to create and load dispense protocols. Using dispense protocols can help you work faster and more efficiently, particularly if you frequently use the same hardware setup or plate design. Protocols essentially tie together a reagent configuration file and a dispense list (see Creating and Using Reagent Configurations and Dispense List for more information). If your protocol was created by importing an external dispense data in .CSV format, the protocol will be also linked to the .CSV file, allowing you to reload your dispense data from an updated .CSV file.
To create a protocol:
Design a plate dispense and assign each reagent to a chip, as normal.
Go to the File menu, point to Protocols, and then select Save.
Note: If you have not yet saved the dispense list and/or the reagent configuration files, you will be prompted to do so before you can save the protocol.
Browse the file directory where you want to save your files.
Type a name for the protocol in the File Name box, click Save, and then click Yes when the confirmation message appears.
You have successfully created your protocol. Please note that you can open an existing dispense list and create a protocol from that point of the guide. To load the protocol for future use, follow the steps below.
To load a protocol:
Go to the File menu, point to the Protocols menu, and then select Load.
If you do not find your protocol files, click the Browse button
next to the Current Path box and locate your protocol files.
Select the protocol from the list in the Select Protocol box.
Optional: You can select or deselect the Reload volume data from .CSV option if you have previously imported the dispense list protocol from the Importing Dispense Data menu. This option will load the dispense data found in the .CSV file, disregarding any changes previously made in MANTIS.
Click Load Files if you want to load both the hardware setup and the dispense design.
Protocol List Window
Note: If you would like to revert a previously imported dispense to its original reagent and volume data, see Reloading Dispense Data.
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