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How to Run Buffer Exchange

 

  1. Tap AUTO PROFILE on the Home Screen and select BUFFER EXCHANGE tab.

  2. Select the conical tube capacity options from the Left Conical.

  3. Select the MWCO from two chip options MPES or RC according to your preference and select the desired kDa.

  4. Input the Starting Volume and Final Volume. The rest of the parameters will adjust automatically. You can customize the value of other parameters as desired.

    The Buffer Exchange Tab

  5. OPTIONAL: Click on the Exchange Volume calculator and select the extent of the buffer exchange by inputting the percentage required. It will calculate the volume of the Buffer B required for the exchange.

    The step size indicates the volume of Buffer B exchanged at each step. You can reduce this volume according to the requirement.

    Buffer Exchange Calculator

  6. Click the ADVANCED SETUP EXPLORER to change the pressure settings.

  7. Tap RUN NOW to run the profile or SCHEDULE RUN to create a schedule.

 

 

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