FRAP Imaging (Optional)

FRAP (Fluorescence Recovery After Photobleaching) imaging is available as a standalone Benchtop imager or can be added to a ROCK IMAGER 1000 system. FRAP measures membrane protein mobility in lipids. High protein mobility and fast diffusion rates correlate well with crystallization conditions. The system utilizes an automated high throughput LCP-FRAP assay to pre-screen membrane protein crystallization conditions.

FRAP was developed in collaboration with Vadim Cherezov (TSRI) and the JCIMPT center led by Ray Stevens and supported by the NIH Common Fund in Structural Biology.

 

Key Features

 

How Does FRAP Work?

The LCP-FRAP imager bleaches the dye-labeled protein in LCP with a laser pulse. Then, a sequence of post-bleaching images is recorded. The image processing function in the imager software measures the pixel intensity inside the bleaching spot. Protein mobility parameters are extracted from fitting the pixel intensity recovery curve with either single component or double component 2-D diffusion equation.

The LCP-FRAP imager is fully automated to conduct imaging and data analysis for LCP plates.

The diagram below depicts the light path involved in the FRAP imaging method.

The Diagram of the FRAP Imaging Method

 

Related Topics


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