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SONICC Best Practices
Here are some helpful hints for getting the best results from your SONICC imager.
- If you are using SONICC Benchtop and you want to add a new plate type to your library with the plate type editor, you must check the box next to "Enable for this plate type" (see screenshot below) or any experiment that uses that plate type will not be imaged with SONICC.
SONICC Plate Type Editor
- Imaging settings override any exposure, binning and laser power settings on the Plate Type Editor, so if you want to make changes to these settings do so in the ROCK MAKER imaging setting, not the Plate Type Editor.
- Use the same z-range defined for the visible settings with DOF limited. The depth of field for SONICC is around 50 um, therefore the recommended maximum is steps of 50 um. The DOF does not change as a function of zoom.
- For LCP it is very important to limit the imaging range to within the LCP drop so that the laser does not focus on the glass slides, otherwise strong interference is generated. Use drop location and autofocus in visible, and then to do a z range of 3 slices below and 2 above the focus level found in SONICC.
- If no sample is present, it is expected to see ~20 pixels with a count of 1 and all others with a count of 0. Check by leveling from 0-1 and should see around 20 white pixels for every frame.
- Urea and tryptophan are very bright and should see clear contrast with leveling from 0 to 30.
- Typical leveling for protein samples would be from 0 to 5 for SHG and 0 to 20 for UV-TPEF.
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RIC-V38R119
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