Grid Layers

 

Grid layers allow you to vary the concentrations and pH levels of ingredients in incremental steps along the rows and columns of a layer, as well as from one layer to another through the use of sub-layers.

The idea behind a grid layer is that through experimentation you have found a combination of ingredients that has started to produce crystals, and you want to optimize that condition in an organized, controlled fashion. The best way to do this is to use a grid layer to vary each of the ingredient combinations to a certain degree until you find the optimal crystal-forming conditions.

If you have ingredient data in JMP, Excel, or other Laboratory Information Management Systems (LIMS), you can create a .CSV file of ingredients you want to use. Then, use the DoE button to import the ingredient conditions from the .CSV file to ROCK MAKER a grid layer. For more information, see Importing Ingredients Using the DoE Tool.

Edit Grid Layer Side Panel

 

After you create a grid layer, you can add one or more sub-layers to it. A sub-layer is a copy of a grid layer. While the rows and columns of the original grid allow for methodical variation in two dimensions, the addition of sub-layers allows for a third dimension of variation. A sub-layer grid will contain the same ingredients found in the original grid, and it will be of the same size as the original. When you resize one sub-layer, all related sub-layers will be resized as well.

 

How Ingredients are Varied on a Grid

Grid layers automatically vary the concentration or pH of an ingredient. Type a start value and a stop value, and then specify where you want the variation to occur (for example, along a row or down a column) by selecting a value from the Vary list.

ROCK MAKER provides the following options for varying concentrations:

None

When you select None, there will be no variation. The start value will be used in all wells of the grid.

Row

When you select Row, variation will occur along each row in equidistant steps beginning with the start value and ending with the stop value.

Vary Along Row

Column

When you select Column, variation will occur along each column from the start value to the stop value.

Vary Along Column

Sub-layer

When you select Sub-layer, variation will occur from each sub-layer to the next. Every well in each sub-layer will be assigned the same value, which will increment in equidistant steps between the start and stop values.

Vary Along Subgrid

Well Number

When you select Well Number, variation will occur from well to well. To determine the increment, ROCK MAKER looks at the total number of wells in the grid, as well as all wells in any sub-layers. The first well of the grid will receive the start value, and the last well of the grid or sub-layer will receive the stop value. All wells in between will be incremented in even steps. The following example illustrates this using a single grid.

Vary Along Well Number

The following example illustrates variation along well numbers across a grid and one sub-layer. Note that variation continues from the last well of the first grid to the first well of the sub-layer.

Variation Along Well Numbers Across a Grid and One Subgrid

Custom

Custom variation allows you to define the volume or concentration for each well using a spreadsheet editor. When you are done making changes, save the file and close the spreadsheet editor. The Canvas will update accordingly.

 

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