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A dispense delay can be applied to your experiment when you want TEMPEST to pause at a particular point during dispense list execution.
There are three delay priority types available: Timer, UserInput, and Smart Batch.
Timer — You set a finite delay time, after which time TEMPEST continues the dispense process automatically. If you select this option, you will need to set the delay time in hh:mm:ss format. This option is useful when you are dispensing a plate and you want TEMPEST to pause for a specific amount of time.
UserInput — TEMPEST pauses the dispense process and waits for you to take further action. This option is useful if your delay time is not definite but you want TEMPEST to pause at a particular point during the dispense. You can either continue or cancel the dispense process at that point.
Note: The Timer and UserInput delay provide you with the Pre-dispense after delay option, allowing the TEMPEST system to automatically do a pre-dispense after the delay. This option is only applicable when the nozzle has already been primed. It is especially useful to ensure more accurate dispense after long delay between reagents, which might cause some reagents to evaporate.
The Pre-dispense After Delay Option in the Delay Settings Window
Smart Batch — This option enables you to increase dispense efficiency by sequentially dispensing multiple reagents into multiple barcoded plates, and then restack the plates to the unloading dock. TEMPEST will group multiple barcoded plates into a single batch and automatically calculate the number of plates in a batch, depending on the defined the delay time in hours, minutes, or seconds.
For example, you have designed a dispense of 10 plates, where two reagents A and B are dispensed with a 5 minute delay in between. TEMPEST will first dispense reagent A to the first plate, then restack it to the loading dock. Then, TEMPEST will load the second plate, dispense reagent A, and restack it, continuously to the next plates until the delay time is over. After all plates are dispensed with reagent A, TEMPEST will do the same for reagent B, by first unloading all plates back to the loading position and then dispensing them. To enable the use of plate stackers with your TEMPEST, see Stacker Settings in the Options menu.
Smart Batch Delay Options in the Delay Settings Window
TEMPEST will automatically add the dispense time estimation to the Estimated Run Time after you set the delay period.
See Adding a Dispense Delay to learn how to add a dispense delay to your dispense list.