Designing a Dispense

Once you have set up the hardware, you will use the TEMPEST control software to either create a new dispense list, open a dispense list, and design a dispense. Before designing a dispense, you have to create a new dispense list or open an existing one. The TEMPEST software provides you with some dispense list templates. However, you can also create your own dispense, as shown in this tutorial.

 

Prerequisites

 

To design a dispense:

  1. Choose from the following options:

  2. In the Add Reagent To Dispense List window, define the Reagent Name. Then, type the Volume per well and click OK.

  3. Begin designing your plate dispense with the plate designer in the Dispense List Designer panel. Click and use your keyboard or mouse to select a group of wells and select a pattern from the Selection group icons below the plate designer:

  4. To add additional reagents to the dispense list, click the Add Dispense List Item button and repeat steps 2 and 3.

  5. When you are done, click the Save Dispense List button to save the dispense list.

  6. Then, choose from the following options:

  7. Define the Dispense List Name, and select the file type from the Save as type list from the following options:

  8. Click Save when you’re done.

    Note: If you want to categorize an unassigned dispense list, open the desired dispense list from the Home category and modify it as needed. Then, click the small down arrow next to the Save Dispense List button , and select Save As Dispense List. Select one of the available categories under the Category List, or click Add New Category to create a new category. Define a new dispense list name in the Dispense List Name box and Save as type field, then click Save As when you’re done,

  9. Drag each reagent from the Dispense List to the appropriate location on the Device Control panel.


Reagents on the Dispense List Designer

  1. Set the input type for each chip by clicking the button at the top of each chip (refer to image below). This button is a toggle; to change it to from pipette tip to tube input or vice versa, click the button.

Input Buttons

  1. Once you have added all reagents to the dispense list and assigned them to their positions, proceed to the next step, priming the chips.

 

Related Topics


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