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Default Volume and Tubing Length Recommendation

The following values for your chip input settings are a good starting point to design your dispense for the first time, but may require some changes depending on your application and length of tubing. It is recommended for you to cut your input tubing to a length of 45 cm.

All chip input settings can be configured from the Input Control panel. Click the Input button to display the Input Option dialog box, and then specify all the chip input settings as needed. If you are using a tube, refer to the default values under the Tube Input Source Type section. If you are using a pipette tip, see the Pipette Input Source Type section.

 

Tube Input Source Type

 

Tube - Low Volume Chip Type*

 

Input Source Type Tube (45 cm)
Chip Type Low Volume High Volume Low Volume PFE High Volume PFE Low Volume 3PFE High Volume 3PFE High Volume CF
Prime Volume (µL) 100 200 100 200 100 200 250
Pre-Dispense Volume (µL) 0.6 6 0.6 6 0.6 6 -
Recovery Volume (µL) 100 200 100 200 100 200 -
Wash Volume 1 (Station 1) 150 150 150 150 150 150 -
Wash Volume 2 (Station 2) 150 150 150 150 150 150 -
Wash Volume 3 (Station 3) 150 150 150 150 150 150 -

Default Volume Values for Tube Input Source Type

 

Pipette Tip Input Source Type

 

Pipette Tip - Low Volume PFE Chip Type*

 

Input Source Type Pipette Tip
Chip Type Low Volume High Volume Low Volume PFE High Volume PFE Low Volume 3PFE High Volume 3PFE
Reagent Volume (µL)** 1000 1000 1000 1000 1000 1000
Prime Volume (µL) 6 16 6 16 6 16
Pre-Dispense Volume (µL) 0.6 6 0.6 6 0.6 6
Recovery Volume (µL) 6 16 6 16 6 16
Wash Volume 1 (Station 1)

150

150 150 150 150 150
Wash Volume 2 (Station 2)

150

150 150 150 150 150
Wash Volume 3 (Station 3)

150

150 150 150 150 150

Default Volume Values for Pipette Tip Input Source Type

 

*We provide these screenshots for general reference. The default values recommendation vary depend on the input source type and chip type selected.

**Available when the Pause for Reagent Refill option is set to Enable.

 

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