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Designing a Dispense

Once you have set up the instrument, you can either load an existing dispense list or design a new dispense.

 

Prerequisites

 

To design a dispense:

  1. Choose from the following options:

  1. In the Add Reagent To Dispense List window, define the Reagent Name and type the Volume per well, then click OK. If you want to add additional reagents, click Add More.

Add Reagent to Dispense List

  1. Use dispense list designer to enter and/or edit reagent volumes:
  2. To add additional reagents to the dispense list, click the Add Dispense List Item button and repeat steps 2 and 3.

  3. When you are done, click the Save Dispense List button to save the dispense list.

  4. Then, choose from the following options:

  5. Define the Dispense List Name, and select the file type from the Save as type list from the following options:

  6. Click Save when you’re done.

    Note: If you want to categorize an unassigned dispense list, open the desired dispense list from the Home category and modify it as needed, then click the Save As Dispense List button . Select one of the available categories under the Category List, or click New Category to create a new category. When you're done, click Save.

  7. Click the Input button next to each reagent input. Select the Chip Type (Low Volume, High Volume, High Volume PFE, Low Volume PFE, High Volume 3PFE, Low Volume 3PFE, or High Volume Continuous Flow chip), and enter the Prime Volume (optional), Primary Wash Volume, Secondary Wash Volume (optional), Recovery Volume, and Pre-Dispense Volume. See Default Volume and Tubing Length Recommendation.
  8. Drag the reagent name from the Dispense List to either the text box next to the chip position, or the chip position on the image of the MANTIS. OR click the Assign To Input button to assign all reagents from the Dispense List at once.

Drag Reagent to the Device Control Panel

 

Assign a Dispense List of Reagents to Chip Positions

Note: If the dispense list has more reagents than the amount of the chip stations, the Assign To Input button will not work. The amount of reagents should be less or the same as the amount of the chip stations.

 

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