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Once you have set up the instrument, you can either load an existing dispense list or design a new dispense.
Choose from the following options:
Create a new dispense list:
Open an existing dispense list.
Note: MANTIS will automatically list any unassigned dispense lists in Home. Expand the Home folder to see all of the available dispenses.
Open the Desired Dispense List
Add Reagent to Dispense List
Click and drag your mouse over a group of wells to select them, or select a pattern from the Selection group under the plate designer.
For a constant volume, type in a volume and press Enter on your keyboard.
To use a gradient design, click the Grid button , and choose a horizontal gradient, a vertical gradient, a forward diagonal gradient, or a backward diagonal gradient.
To add a backfill volume, click the Grid button , and then click the Backfill button .
Gradient Design in the Dispense List Designer
To add additional reagents to the dispense list, click the Add Dispense List Item button and repeat steps 2 and 3.
When you are done, click the Save Dispense List button to save the dispense list.
Then, choose from the following options:
To assign the Dispense List to an existing category, select the desired category from the list.
Save Dispense List Window
To add a new category, click on the name of the category under which you would like to add the new category label. Click New Category, and type a name in the Category Name box. Then click Add to add the new category to the category list.
Creating a New Category
Define the Dispense List Name, and select the file type from the Save as type list from the following options:
Click Save when you’re done.
Note: If you want to categorize an unassigned dispense list, open the desired dispense list from the Home category and modify it as needed, then click the Save As Dispense List button . Select one of the available categories under the Category List, or click New Category to create a new category. When you're done, click Save.
Click the Input button next to each reagent input. Select the Chip Type (Low Volume, High Volume, High Volume PFE, Low Volume PFE, High Volume 3PFE, Low Volume 3PFE, or High Volume Continuous Flow chip), and enter the Prime Volume (optional), Primary Wash Volume, Secondary Wash Volume (optional), Recovery Volume, and Pre-Dispense Volume. See Default Volume and Tubing Length Recommendation.
Drag the reagent name from the Dispense List to either the text box next to the chip position, or the chip position on the image of the MANTIS. OR click the Assign To Input button to assign all reagents from the Dispense List at once.
Drag Reagent to the Device Control Panel
Assign a Dispense List of Reagents to Chip Positions
Note: If the dispense list has more reagents than the amount of the chip stations, the Assign To Input button will not work. The amount of reagents should be less or the same as the amount of the chip stations.
On the Dispense Setting tab, type the amount of plates you want to dispense in the Number of Plates field.
Number of Plates Field