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MANTIS volume options/dispense pattern options are available in the Volume Fill Type list on the Dispense List Designer. When you design a dispense with MANTIS, you can choose to fill the selected wells with a constant volume; vary the volume across the plate in a number of ways using the Gradient feature, backfill with a specific reagent to create a uniform total volume across the plate, or scale in order to multiply the volumes of the wells by a common factor.
Read more about the available options:
When you select a constant volume, the MANTIS will apply the same total volume to the selected well(s).
When you select a horizontal gradient fill, the volume will increase gradually from left to right across the plate.
When you select a vertical gradient fill, the volume will increase gradually from the top of the plate to the bottom of the plate.
When you select a forward diagonal gradient fill, the volume will increase gradually from the top-left corner of the plate to the bottom-right corner of the plate.
Forward Diagonal Gradient
When you select a backward diagonal gradient fill, the volume will decrease gradually from the bottom-left corner of the plate to the top-right corner of the plate.
Backward Diagonal Gradient
Backfill is useful if you are using varying volumes for different ingredients, but you want each well to have the same total volume. If you select Backfill volume, the MANTIS will fill all the wells to the same total volume using various amounts of the selected reagent.
The backfill volume should be the final item on the dispense list. If you must add an additional reagent after you have already added a backfill volume, delete the backfill volume item on the dispense list and add it again after you have defined the additional reagent(s). The software is not programmed to recalculate backfill volumes if new items are added to the dispense list.
The Scale feature is useful if you want to change the volume of all or some of the wells on your plate. It acts as a multiplier for the currently entered volume. For example, if your group of selected wells has a volume of 20, and you enter 0.5 as the scale number, the volume in the selected wells will be reduced to 10.