Creating a Profile

PULSE allows you to create a profile consisting of desired experiment settings for concentrating sample or performing the diafiltration process. You can set up a profile for the following experiment types:

Concentrate only
Concentration + Buffer Exchange and Buffer Exchange Only

Concentrate only

To create a new profile for concentration:

On the Home Screen, open the EXPLORER menu and tap the ADD button in the lower-left corner of the EXPLORER window.
Type a name for your new profile by tapping on the pencil button.
Type the Starting Volume and Starting Concentration based on the sample you’re using, and then type the desired Final Volume. The Estimated Final Concentration will be automatically generated after you have set the other values.
OPTIONAL: If you need to concentrate sample more than 50 mL, select ON in the >50 mL Volume option and type the volume in the Starting Volume in 2nd Tube option. The 1st tube is assumed to have 50 mL.
In the Buffer Exchange option, select NONE if you want to purify the sample without exchanging buffer.
Select the correct chip valve in the Chip Valve option.
Select the Membrane Cutoff Size that matches the chip you’re using from the Membrane Cutoff Size drop-down list.
OPTIONAL: You can type some notes about your experiment in the NOTES field in the lower-right corner of your screen.
Tap SAVE.

Concentration + Buffer Exchange and Buffer Exchange Only

To create a new profile for concentration and buffer exchange:

On the Home Screen, open the EXPLORER menu and tap the ADD button in the lower-left corner of the EXPLORER window.
Type a name for your new profile by tapping the pencil button.
Type the Starting Volume and Starting Concentration based on the sample you’re using, and then type the desired Final Volume. The Estimated Final Concentration will be automatically generated after you have set the other values.
When performing buffer exchange you can not concentrate more than 50 mL, leave >50 mL Volume OFF.
In the Buffer Exchange option,
1. Select DISCRETE if you want to dilute the solution and re-concentrate it in one or more cycles.
2. Select CONTINUOUS if you want to exchange buffer continuously while holding sample at a set volume.
Type the Exchange Count if applicable,
Type the value in the Sample Volume to Start option. This is the sample volume at which buffer exchange will begin
Type the value in the Buffer Volume to Use option.
Type the value in Total Buffer Volume option.
Select the correct chip valve in the Chip Valve option.
Select the Membrane Cutoff Size that matches the chip you’re using from the Membrane Cutoff Size drop-down list.
OPTIONAL: You can type some notes about your experiment in the NOTES field in the lower-right corner of your screen.
Tap SAVE.

More Options

You can configure more profile settings to meet your preferences by tapping the MORE button. There you can find a list of advanced settings that only apply to this profile, such as vacuum and pressure values, diaphragm timing, liquid recovery, and so on. Make sure that you understand these options before making any changes.

Option	Description	Recommended Value	Default Value
Diaphragm Vacuum Pressure	Determines the diaphragm vacuum pressure value.	-11 - 0 psi	-10 psi
Diaphragm Pressure	Determines the diaphragm pressure value.	30 - 80psi	50 psi
Diaphragm Timing	Determines the timing of the diaphragm.	100 - 1000 ms	225 ms
Recovery of Liquid	Determines whether to use air, buffer, or both air and buffer to recover the remaining solution from the filter chip.	-	None by default
Skip Priming Sequence	Set this option to skip priming when PULSE runs a profile.	-	Off
Operating Pressure	Pressure value that must be reached by the system in order to be able to run a concentration.	10 - 40 psi	20 psi
Valve Timing	Determines the operational timing of the valve.	10 - 50 ms	25 ms

Editing a Profile

You can modify saved profiles in the EXPLORER window whenever you want to make changes to the existing profile settings, such as the starting volume, final concentration, final volume, and so on.

To edit a saved profile:

On the Home Screen, tap the EXPLORER menu, and then tap a profile you want to edit.
Tap the EDIT button and change the options you want to edit.
Tap SAVE.

PULSE Video Tutorials

You can also watch the videos below for a quick overview of how to set up various experiment types.