Connect the PULSE hardware to a power outlet using the power cable.
Find the power button located on the back of the PULSE hardware and turn it on.
Open the handle clamp.
The PULSE Hardware with the Handle Clamp Opens
Open the waste door, insert the waste tank, and then insert the waste slide into the slot behind the tube opening on the tube mount.
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The Waste Tank and Waste Slide Are Attached
Fill the sample tube with the sample or your carrier reagent, then attach the tube cap securely. This tube should go in the left input.
A Sample Tube Is Attached on the Left Input
Fill the buffer tube with the buffer of your choice, then attach the tube cap securely. This tube should go in the right input.
A Buffer Tube Is Attached on the Right Input
Attach the desired chip with the diaphragm side facing the front of the instrument. You will hear a click when the chip is pushed in all the way. PULSE filter chips come with six membrane cutoff sizes: 3, 5, 10, 30, 50, and 100 kDa. Choose the chip that corresponds to the size of the sample you are using. Typically you should use a cutoff size that is less than half the size of the sample or target of interest you want to concentrate.
The Filter Chip Is Attached
Close the handle clamp carefully until it is flush with the surface. If there is resistance, please make sure that the filter chip is pushed all the way into its slot and the tubes are seated properly.
The Handle Clamp Is Closed
