Concepts

Profile

A profile is a set of experiment settings that can be used for concentrating a sample or performing diafiltration or buffer exchange. You can set up a profile from the EXPLORER menu on the home screen. Learn more about Creating or Editing Profiles.

Prime

The priming process is the first step of concentrating your sample solution. The system will fill the filter chip with buffer to reduce air bubbles formed during the concentration process.

Concentrate

The concentrating process will be performed after the priming process is done. The sample will be filtered using tangential flow filtration across the membrane in the filter chip.

Buffer Exchange

This is an optional process if you want to perform buffer exchange or desalting with concentration. The reagent type can be the same or different as the carrier reagent.

Diafiltrate

Using the same buffer solution as the original sample, this process allows you to dilute and re-concentrate sample to remove more of the non-target molecules in the sample solution.

Liquid Compatibility

The PULSE instrument is compatible with all aqueous sample and buffer solutions. You can use the following reagents to clean PULSE instrument, in order of preference:

0.1 up to 1 M of NaOH (sodium hydroxide)
6 M Guanidinium chloride
8 M Urea

Important: All cleaning solutions must be filtered through a 0.22 μm pore filter or similar.