Concepts

Profile

A profile is a set of experiment settings that can be used for concentrating a sample or performing buffer exchange. You can set up a profile from the EXPLORER menu on the home screen. Learn more about Creating or Editing Profiles.

Prime

The priming process is the first step of concentrating your sample solution. The system will fill the filter chip with buffer to reduce air bubbles formed during the concentration process.

Concentrate

The concentrating process will be performed after the priming process is done. The sample will be filtered using tangential flow filtration across the membrane in the filter chip.

Buffer Exchange

This is an optional process if you want to perform buffer exchange or desalting with concentration. The reagent type can be the same or different as the carrier reagent.

Liquid Compatibility

The PULSE instrument is compatible with all aqueous sample and buffer solutions. You can use the following reagents to clean PULSE instrument, in order of preference:

0.1 up to 1 M of NaOH (sodium hydroxide)
6 M Guanidinium chloride
8 M Urea

Important: All cleaning solutions must be filtered through a 0.22 μm pore filter or similar.

SEQUENCE RUNNER

You can create and run a sequence based on the number of cycles and time (in minutes) in the SEQUENCE RUNNER menu. To learn more about each part in the SEQUENCE RUNNER menu, read the explanation below. To create a sequence, see Creating a Sequence in the Sequence Runner.

The Sequence Runner Menu

No	Item	Description
1	ADD NEW	Add a new cycle card.
2	Help Button	Tap to show the SEQUENCE RUNNER help page.
3	REPEAT ALL	Repeat the sequence for x time(s).
4	Delete All Button	Delete all cycle cards.
5	Grip Indicator	Drag and drop the grip indicator to move the card.
6	ON/OFF Switch	Select ON to include the cycle during sequence execution. Select OFF to skip the cycle during sequence execution.
7	Cycle Selector	Tap and select one of the cycles in the list.
8	Cycle Time Settings	Determine the cycle iteration or cycle execution time.
9	Pressure Switch (HI/LO)	Select pressure settings: High pressure or Low pressure.
10	Cycle Settings Button	Open the cycle settings.
11	Delete Cycle Button	Delete a cycle card from the list.