Before you set up the µPULSE hardware, you’ll need to understand the names of its components and where each component is located on the system.
µPULSE - Front View
The µPULSE New Tubing Block
The µPULSE 50 mL Sample Tube and Buffer Tube
The µPULSE New Waste Slide
The µPULSE New Tube Adapter
µPULSE Tube Stand
The touch screen is where you can interact with µPULSE. Here you can control the instrument, create and run your experiments, and so on.
The chip cover securely holds the chip in place over the tube mount. To open it, rotate the latch until the chip cover releases.
The µPULSE filter chips come with six membrane cutoff sizes: 5, 10, 30, 50, 100, and 300 kDa. Filter chips can be purchased from the FORMULATRIX online store at https://formulatrix.com/accessories/. See also, Chip Overview.
The waste slide transfers waste liquids from the chip to the waste tank.
The µPULSE waste door keeps the waste tank covered. You can easily check the waste tank capacity through the window in the waste door.
The waste tank is used to deposit waste liquids from the chip. The waste tank can hold up to 125 mL of liquid.
The tubing block connects the chip to the liquid tubing for aspirating and returning the sample and/or buffer to the 50 mL tube. It should be washed after each use if it will be reused. See Cleaning Chips and Tubing for more information.
The left input of the µPULSE is for the sample tube, and the right input is for the buffer tube. Any common 50 mL tube will work with µPULSE.
µPULSE - Back View
The Ethernet port is used to connect the µPULSE instrument to the Internet through a wired network, so that you can update the µPULSE software and monitor the status remotely.
The USB port is used for updating the µPULSE software manually if an Internet connection is not available. It may also be used by support for debugging or troubleshooting purposes.
The power button turns the µPulse On or Off. To turn off the device, it is preferred to shut down the system by tapping the SHUTDOWN button on the Home Screen. Pressing the power button once while the device is on will preform a normal shutdown the same as through software. Pressing and holding the power button for more than 5 seconds and releasing it will preform a "hard" shutdown.
The power port is where you plug in the power cable. See also, Electrical Requirements.
µPULSE - TFF System filter chips come with six membrane cutoff sizes: 5, 10, 30, 50, 100, and 300 kDa.
Filter Chip Overview
Each filter chip consists of the following components:
Membrane: The membrane is what separates the sample (retentate) from its carrier reagent (permeate) which flows to the waste tank.
Diaphragm: The diaphragm pumps the liquid through the filter chip.
Liquid Layer: The liquid layer is the channels through which the liquid flows.
Air Layer: The air layer is the channels through which air flows to actuate the diaphragm.
The µPULSE - TFF cleaning chip is exclusively used for cleaning the tubing.
Cleaning Chip Overview
Each cleaning chip consists of the following components:
Diaphragm: The diaphragm helps the liquid flow through the cleaning chip.
Liquid Layer: The liquid layer is the channels through which the liquid flows.
Air Layer: The air layer is the channels through which air flows to actuate the diaphragm.
PTFF-V20R221 |