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The µPULSE allows you to create a profile consisting of desired experiment settings for concentrating sample or performing buffer exchange. The system tracks volume as it runs sequences based on the values you have entered when designing a profile.
To set up a new profile, tap AUTO PROFILE on the Home Screen. Then choose between these 3 options:
Tap the CONCENTRATE tab if you want to concentrate a sample 50 mL or less.
Tap the CONCENTRATE > 50 ML tab if you want to concentrate more than 50 mL of sample.
Tap the BUFFER EXCHANGE tab if you want to exchange buffer solution for another from the buffer (right) tube. The reagent type can be the same or different as the carrier reagent.
The Auto Profile Menu
Tip: Tap the Help button to get acquainted with the software and learn about what you can do in the AUTO PROFILE menu.
To create and run a concentrate profile, follow the instructions below:
Note: The final concentration or final volume will be calculated based on the other inputs.
Setting up the Concentrate Profile
Tap the CONCENTRATE > 50 ML tab.
Fill in the parameters for Starting Volume of Tube 01 and 02, then the Final Volume.
Setting up Concentrate > 50 ML Profile
Then, continue to step 2.
The Buffer Exchange Setup Menu
Click on the exchange volume calculator and select the extent of the buffer exchange by inputting the percentage required. It will calculate the volume of the Buffer B required for the exchange.
Buffer Exchange Calculator
The step size indicates the volume of buffer B exchanged at each step. You can reduce this volume according to the requirement.
Continue to step 2 to load the saved profile for buffer exchange.
Click EDIT to change settings.
In the General Setup tab, the Safety Threshold can be adjusted. You can also choose the priming option between BUFFER or SAMPLE and define the Priming Volume.
Click the CYCLE SETUP tab to to adjust the diaphragm pressure, return valve pressure, and fill/empty time.
Note: Tap the Edit button to adjust Return Valve Pressure.
Advanced Setup Menu
Tap SAVE SETUP AS to save the customized settings.
Click LOAD SETUP to use the saved settings.