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Setting Up the LC3 for a Dispense

Prerequisites

• You have the LC3 option, along with the carousel and a locating plate.
• You are familiar with the component names of the LC3 instrument (see LC3 High Capacity Chip Changer).

To set up the LC3 for a dispense:

1. Position the MANTIS on the center of the locating plate by lining up the pin slots under the MANTIS base with the locating plate’s pins.

2. Position the LC3 on the pins on the locating plate by lining up the pin slots under the LC3 base with the locating plate’s pins.

   

   Left Locating Plate

3. Position the carousel on top of the LC3.

   

   An Example of the Instrument Setup Using Left Locating Plate

4. Put the MANTIS chips into the chip changers.

5. Attach the reagent tubes in the tube holders or pipette tips to the inputs at each chip.

6. Put your plate on the MANTIS deck.

   

   MANTIS With a 16-Chip Carousel

7. Fill the wash container(s) with distilled water or wash solution (if you are using wash solution, you must follow the wash solution with a water rinse). Make sure that the wash container(s) are properly placed in the wash stations located at the back sides of the MANTIS.

Related Topics

• LC3 High Capacity Chip Changer
• Designing a Dispense
• Designing a Multi-Plate Dispense

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