The Dispense List Designer is where you create or import a dispense list and plate dispense design. When opening a dispense list, some reagents will automatically be displayed in the Dispense List table. You can change the dispense priority order by typing the number (either 1 or 2) next to the reagent in the Priority column or dragging the reagent to the desired position in the Dispense List table. Number 1 means that the particular reagent will be dispensed first and number 2 indicates the reagent will be dispensed second.
At the left of the Dispense List Designer is the Dispense List, which is a list of reagents to be used in a particular plate dispense. At the right of the Dispense List is a graphical view of your plate design. This area is where you design the dispense using the reagents in your Dispense List.
Dispense List Designer
There are buttons along the top of the Dispense List that enable you to create a new dispense list, open an existing dispense list, save a dispense list (or save the dispense list with actual dispense volumes), add a new reagent to the dispense list, add a delay, delete a reagent/delay, clear a dispense list, or duplicate a selected item on the dispense list.
The Dispense List Buttons
At the bottom of the dispense list you will see a box of information that changes based on which reagent is highlighted in the dispense list. This table shows you the reagent's name, volume per well, its liquid class, dispense mode, barcode, and the total required volume. You can change these items if they are shown in bold text. To see your options, click anywhere in the text field of the bold text, then click the down-arrow to display the options.
Reagent Information Box
The Plate Designer is used to design the plate dispense. You can click on a single well to select it; click and drag to select an area of wells, or hold down the Ctrl key on your keyboard while clicking wells to select non-adjacent wells.
Plate Designer
The Plate Designer has its own toolbar buttons that enable you to switch between Designer View, Summary View, Volume Error View, and Actual Volume; change the volume fill type, copy and paste selected wells, clear all wells, undo and redo design actions; change the fit of the virtual plate on your screen; and edit a dispense design using a spreadsheet editor.
While most of your work will be done using Designer view (the default plate view), you can select other views by clicking the View Dispense List Editor button from the toolbar at the top.
Plate Designer Toolbar Buttons
The Volume Fill Type button opens a list that shows you different options for how to vary the volume across a selected area. The default setting is Constant Volume, but you can select either Horizontal Gradient, Vertical Gradient, Forward Diagonal Gradient, Backward Diagonal Gradient, Backfill, or Scale.
For more information about any of the types of fills, please see Volume Options: Constant, Gradient, Backfill, Scale.
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