You can easily modify the pipetting settings through the Pipetting Parameters feature that can be accessed and managed through the Pipetting Profile menu. By default, the FLO i8 provides you with the Factory Profile and Factory SD (Serial Dilution) Profile in the Pipetting Profiles Explorer page, that you can use in your experiment.
Important: Both factory profiles in the Pipetting Profiles Explorer page can be used in the protocols, but cannot be modified, removed, or copied to other users.
Pipetting Profiles Explorer Page
There are 2 pipetting profile categories:
Regular Pipetting Profile — The regular pipetting profile can be used with the Simple Transfer and Experiment Designer pipetting steps. There are four tabs in the Regular Pipetting Parameters settings: Tips, Mixing, Optimization, and Advanced.
Regular Pipetting Parameters Settings
Serial Dilution Pipetting Profile — For Serial Dilution pipetting steps, use the Serial Dilution Pipetting Profile settings. There are three tabs in the Serial Dilution Pipetting Parameters settings: Serial Dilution Settings, Sample Pipetting Parameters, and Diluent Pipetting Parameters.
Serial Dilution Pipetting Parameters Settings
Each tab in the Pipetting Parameters Settings includes predefined Aspirate and Dispense settings, which determine the pipetting settings during both operations. For more information, see Using the Pipetting Parameters.
Choose from the following options to access the pipetting parameters.
From the Pipetting Profiles Explorer page
Click the Application button
, select the Explore menu, and then click Pipetting Profile.
In the Pipetting Profiles Explorer page, select a pipetting profile either from the My Regular Pipetting Profile or My Serial Dilution Pipetting Profile section, and click OPEN. For quicker access, double click on the selected profile.
Accessing the Pipetting Parameters in the Pipetting Profiles Explorer
From the Pipetting Profiles Explorer page, you can add a new profile, modify and manage existing profiles. For more information, see Using the Pipetting Parameters.
Within a pipetting step
While authoring or editing a pipetting step in a protocol, open the pipetting parameters from the Pipetting Parameters tab. See the images below to open the tab from the Simple Transfer, Experiment Designer, and Serial Dilution step.
Pipetting Parameters Tab in the Simple Transfer Window
Pipetting Parameters Tab in the Experiment Designer Window
_742x350.png "Pipetting Parameters Tab in the Serial Dilution Window (NO STOCK)")
Pipetting Parameters Tab in the Serial Dilution Window (NO STOCK)
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