[ROCK MAKER Integration] Fragment Screen Dispensing

Starting from version 4.14, NT8 allows you to dispense experiments with fragment screens designed in ROCK MAKER.

Prerequisites:

• You are running ROCK MAKER version 4.11 or newer.

• You have an experiment with at least 1 fragment screen designed in ROCK MAKER. Otherwise, create one by referring to Creating a Fragment Screen.

  Important

  Select one of the options except No Fragment from the NT8: Fragment Layer Protocol dropdown in the Dispensing section of the Info menu of your experiment page. Selecting No Fragment prevents NT8 from detecting your fragment screen.

To dispense a fragment screen:

1. Go to the RM Integration mode.

   

   RM Integration Mode

2. Choose a plate station for the experiment plate (the following example uses Plate Station 1) and enter the experiment barcode number or scan it with a barcode scanner (if it is available). If the plate is not defined in the Plate Type Library, a Warning dialog box appears. To proceed, click the Continue button in the dialog box.

   

   Loading an Experiment

3. Click the plate station where the fragment screen source plate is placed (highlighted in red). The example below uses Plate Station 2 as the fragment screen source plate. Then, select a plate type.

   

   Selecting a Plate Type

   Note

   Each fragment screen experiment requires its own fragment screen source plate. If you upload multiple fragment screens—in one or multiple experiments—you must assign one fragment screen source plate per fragment screen experiment.

4. On the Checklist tab, tick the checkbox to view the instructions that guide you to physically prepare your labware. You can modify the instructions if necessary. Read Checklist Tab for more information.

   

   Checklist Tab’s Checkbox

5. OPTIONAL: Modify the Liquid Class and Dispensing Properties.

   Modifying Liquid Class

   Open the Canvas Tab and modify the Liquid Class for the fragment screen (set to Very High by default). For further details, read Canvas Tab.

   

   Fragment Screen Liquid Class

   Modifying Dispensing Properties

   1. Go to the Experiment Info tab. Click the Edit button in the Dispensing Properties section.

   2. Click Customize to modify the Dispense Protocol. For further details, read Experiment Info Tab and Dispense Protocol.

      

      Customize Button

   3. Tick the Mix Fragment checkbox to modify the Mix Fragment settings. For further details, read Experiment Info Tab.

      

      Mix Fragment Settings

   Return to the Checklist tab once you finish modifying the settings.

6. If everything is set, click the Run Protocol button.

7. Select Yes, run the protocol in the Run Protocol dialog box.

   

   Run Protocol Dialog Box

Related Topics:

• Additive Screen Dispensing

• Seeding Experiments

• Dispense Protocol

• ROCK MAKER® Crystallization Software Integration Mode