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Creating a Pooling Protocol

The Pooling task gathers samples from multiple wells by row or column and dispenses them in one well. Either a 96-well or 384-well microplate can be used as the source or the destination. The instrument will automatically execute the task in sequence, starting from picking up the pipetting tips to ejecting the tips to the waste bin.

Prerequisite:

You have dragged the required labware to the Virtual Tray.

Note: Trough plates cannot be used for pooling tasks.

To add a pooling task:

Click the Pooling button, located on the Switch Panel.
Click and swipe the wells by row or column to define the source.
When the Set aspirate volume per well box appears, type the desired volume in the text field and then press Enter to validate the value. For more details, see the .GIF below.

Defining the Source Well(s) and the Aspirate Volume

Tip: Alternatively, you can scroll your mouse up or down or click the up/down arrow button to define the volume.
Then, click the destination well.

Note: To indicate that samples originate from various wells, the destination well will change to multiple colors.

Defining the Destination Well
OPTIONAL: To modify the pipetting parameters click the Show more dropdown and edit the values as desired. See Using the Pipetting Parameters for more information.
You can add more tasks to the protocol or add mixing, add a delay, user confirmation, and an air gap.

Note: You can easily return to the previous action or reverse the last Undo that you performed by clicking the Undo or Redo button located on the top right section of the page.

Then, click START to run your protocol. See Running a Protocol for more information.

Creating a Pooling Protocol

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