Peristaltic Tubing Harness Replacement Guide

This guide explains how to replace the external peristaltic tubing harness that connects the reservoirs and peristaltic pumps to the stations. Replacement is required if it becomes clogged, damaged, or reaches the end of its service life.

Note

This procedure is written for instruments configured for 3 Concentration Stations and 2 Cleaning Stations. If your instrument is configured for 4 Concentration Stations and 1 Cleaning Station, adjust the steps accordingly.

Required Part

Important

Before installing, inspect the new Peristaltic Tubing Harness Kit for any visible damage or defects.

  • aµtoPULSE Peristaltic Tubing Harness Kit (PN #: 848088B), includes:

Child Item

Part Number (PN)

Image

QTY

25 ft of Extension Tubing

852202A

1 pc

Dispensing needle

826301A

10 pcs

Fitting

817309A

Internal Device Peristaltic Tubing Harness Assembly

838105B

1 pc

Peristaltic Pump Tubing

817792A

9 pcs

Required Tools

  • Tubing cutter (to cut the extension tubing)

  • Nitrile gloves (optional, for hygiene and safety)

  • Flashlight (optional, for better visibility of tubing connections)

Section 1: Preparation

  1. Insert the ends of red and green-tagged tubes into the waste container to collect the liquid from the retraction process.

  2. Retract the pump to drain remaining liquid from the tubing:

    1. Open the Manual Control page from the Tools menu.

    Select Manual Control

    1. Select STATION #, then expand the Pulse Station Movement tab.

    2. Under Retract External Buffer Pump, set the retraction time for 30 seconds to remove any remaining liquid from the station, then click Run runbutton.

      Tip

      Ensure the tubing is dry by checking that only air bubbles are dispensed into the waste container. If liquid remains, rerun the Retract External Buffer Pump or increase the retract duration.

      Retract External Buffer Pump on Concentration Station

      Important

      Before running a retraction on a Cleaning Station, set Drain Waste to OFF before switching to the next station.

      Retract External Buffer Pump on Cleaning Station

    3. Repeat steps b–c to retract the liquid on other stations.

  3. Power off the instrument and pump box before moving on.

  4. Clear the deck and stations of all adapters, labware, and racks. Remove the waste container and buffer reservoirs.

  5. Measure and cut the 25 ft tubing as needed based on the pump and reservoir position. See Section 3 for detailed instructions.

    Tip

    Use the removed Peristaltic Pump Tubing as a measurement guide for the replacement tubing.

Section 2: Removing the Peristaltic Tubing Harness

Note

Retain the tubing in the Waste Drain pumps and cleaning station, as it doesn’t need replacement.

Waste Drain Tubing

Waste Tubing in Cleaning Station

  1. At the Peristaltic Pump Panel:

    Tip

    For quicker tubing removal, open the pump door completely and remove the entire tubing while still connected. Then, proceed to step 2.

    Tubing Set in the Peristaltic Pump

    1. Carefully disconnect tubing from the IN and OUT ports on the Concentration and Cleaning pumps.

    Pulling the Tubing Out

    All Tubings Disconnected

    1. Remove the tubing inside each pump:

      i. Open the peristaltic pump door completely.

      Opening The Peristaltic Pump Access Door

      ii. Pull the tube connector out of the fitting.

      Pull Out The Tube Connector

      Tube Connector Out

      iii. Carefully remove the pump tubing from the housing, then close the pump door.

      Removing The Pump Tubing

  1. Disconnect the tubings accordingly from Stations 1 to 5 as follows:

    1. Remove the waste tubing from the Permeate Port.

      Pulling Off the Waste Tubing

    2. Remove the transfer needle from each station’s right and left sides:

      Note

      The transfer needles are secured with light tension similar to a standard safety pin.

      Warning

      The needle is thin and sharp. Handle carefully to avoid injury or bending.

      i. Detach the needle from the upper holder.

      Needle Detachment from the Upper Holder

      ii. Carefully move the needle to the side as shown below.

      Carefully Move the Needle to the Side

      iii. Gently pull upward to remove the needle from the holder.

      Pull Upward to Remove the Needle from the Holder

      iv. Disconnect the transfer needle from the tubing.

      Disconnect the Transfer Needle from the Tubing

    3. Remove the tubing set from the slots and path as shown below.

      Tubing Slot and Path

      Removing the Tubing Set

  2. Remove the tubing set from the tube clip, then pull the remaining tubing out through the hole on the right side.

Removing Tubing from Tube Clip and Hole

Tip

For easier removal, detach the tube clip from the tubing set. Then, re-attach the clip afterward.

Detaching the Tube Clip with Tubing (Left); Tube Clip is Re-attached (Right)

Section 3: Installing the New Peristaltic Tubing Harness

  1. Insert each color-tagged tubing into the tube clip and pass the ends through the tubing hole. Refer to the image below for correct placement (Station 1–5).

Inserting the Tubing into the Tube Clip

  1. Install the tubing at the stations:

    Important

    Position the tubing properly to keep it clear of the gantry and prevent damage.

    1. Guide the tubing through the tubing path.

    2. Insert each bundle into the holder according to the color tags on the corresponding station.

    3. Route each color-tagged end into the guides and their corresponding parts.

      • Red: Buffer outlet (Left)

      • Yellow: Permeate Port

      • Green: Cleaning solution outlet (Right)

    Inserting Tubing into the Corresponding Slots

  2. Install the transfer needle on the Right and Left sides of the station. Refer to the images below.

    a. Connect the tubing to the needle’s fitting.

    Connect the Tubing to the Needle Fitting

    b. Insert the needle until the angled section rests above the slot.

    Needle Insertion Position Above the Slot

    c. Move the needle over and insert it into the slot.

    Insert the Needle into the Slot

    d. The proper needle placement:

    Proper Needle Placement

  3. Insert the Yellow-tagged tubing into the Permeate Port.

Installing the Waste Tubing

  1. Install the tubing in each peristaltic pump:

    a. Open the pump door, insert the new tubing into the housing until it is securely seated.

    Installing The Pump Tubing

    b. Verify that the pump tubing is fully inserted and the tube connectors are secured in the fittings, then close the pump door.

    Properly Installed Pump Tubing

  2. Connect the tubing to each corresponding port as follows.

    Important

    Verify all tubings are routed through the hole and connected to the correct pumps according to color tags. Incorrect connections may cause liquids to be pumped into the wrong outlet. Use a flashlight if needed for better visibility.

    Color-coded Tubing Connections

    1. Route the Yellow-tagged tubing into the waste container.

    2. Plug the red and green-tagged tubing into the OUT port of the corresponding pump.

      Plug the Tubing into OUT Ports

    3. Plug the extension tubing into each corresponding IN port.

      Plug the Extension Tubing into IN Ports

  3. Verify all tubings are properly connected and secure to prevent leaking.

Section 4: Testing the Installation

  1. Reinstall the waste container and buffer reservoirs.

  2. Place the tube and tube adapter on the stations.

  3. Reconnect the power cable and turn on the instrument. Ensure the door is closed and wait for initialization to complete.

  4. Open the software and go to the Manual Control page.

  5. Select STATION # and expand the Pulse Station Movement tab.Then, define the following parameters:

    1. Select SLIDER FORWARD to move the station forward and prevent liquid from entering the gap between the station’s top and bottom sections.

      Click SLIDER FORWARD

    2. Enter 5 mL and run the External Buffer Pump (you can use either buffer or cleaning solution). If the pump can stop automatically once reaching 5 mL at the station, testing is successful.

      Pumping 5 mL of Liquid on Concentration Station

      Pumping 5 mL of Liquid on Cleaning Station

  6. If a timeout error occurs during circulation, repeat steps 5a-b until it clears. If gurgling or no liquid flows during testing, open and close the pump door or gently push the tubing deeper into the housing.

  7. Inspect all tubing and connections for leaks or mismatched destinations. Liquid should flow only from the correct station’s buffer and/or cleaning outlets, with no leaks along the tubing.

  8. Repeat steps 5-7 for the connections on the remaining stations.