Plate Types Submenu

Accessible from the Setup menu setup, the Plate Types submenu plate_types manages information about various microplate formats used in your experiments. The plate types are available for use when creating screens and designing experiments. By default, ROCK MAKER provides three plate types: 96 Deep Well, 2 mL; 2 Drop Intelliplate LP; and Reference Plate.

Plate Types Submenu

Plate Types Submenu

Plate Definition Section

Plate Definition Section

Plate Definition Section

Each column in the Plate Definition section defines the:

Column

Description

Name

Plate name, e.g. 2 Drop Intelliplate LP, in ROCK MAKER.

Note

The plate type’s name should be unique.

Rows

Number of rows on the plate.

Cols

Number of columns on the plate.

Max Well Vol. (uL)

Maximum volume of each well on the plate.

Def. Well Vol. (uL)

Plate’s default well volume. You may modify this setting for each experiment.

Def. Additive Vol. (uL)

Plate’s default additive volume.

Drops

Maximum number of drops on the plate.

Max Drop Vol. (uL)

A drop’s maximum volume to dispense into a container.

Def. Drop Vol. (uL)

Plate’s default drop volume. You may modify this setting for each experiment.

Def. Seed Vol (uL)

Plate’s default seed volume. You can modify this setting for each experiment.

Type

Plate type: Experiment Plate, Screen Lot Plate, Microfluidics, or LCP.

  • Experiment plate is for a crystallization experiment.

  • Screen lot plate contains pre-mixed well solutions to dispense to the plate used in the experiment.

  • Microfluidic is used for AutoInspex.

  • LCP (Lipidic Cubic Phase) is for the crystallization of membrane proteins.

Enabled

Plate’s state. Clicks to enable or disable a plate type. The disabled plate type is not listed in the Plate Type dropdown of the Info submenu.

Plate Drops Section

ROCK MAKER supports plate types that allow multiple drops in a well. The Plate Drops section lists all drops in a plate type.

Plate Drops Section

Plate Drops Section

Each column in the Plate Drops section displays the:

Column

Description

Drop

Number of drops in the plate type.

Enabled

Drop’s state. Click to enable or disable the drop(s) in the plate type. When a drop is disabled, it is excluded from any experiments created using the selected plate.

Creating a Plate Type

To create a plate type:

  1. Click the Setup menu setup and select Plate Types plate_types.

    Setup Menu and Plate Types Submenu

    Setup Menu and Plate Types Submenu

  2. In the Plate Definition section, click add_plate_type.

    Then, enter the:

    • Plate name in the Name column.

    • Number of rows on the plate in the Rows column.

    • Number of columns on the plate in the Cols column.

    • Maximum volume of each well on the plate in the Max Well Vol. (uL) column.

    • Default well volume in the Def. Well Vol. (uL) column.

    • Default additive volume in the Def. Additive Vol. (uL) column.

    • Maximum number of drops on the plate in the Drops column.

    • A drop’s maximum volume to dispense into a container in the Max Drop Vol. (uL) column.

    • Default drop volume in the Def. Drop Vol. (uL) column.

    • Default seed volume in the Def. Seed Vol (uL) column.

    Select the:

    • Plate type in the Type column.

    • State (enabled or disabled) in the Enabled column.

    If necessary, click:

    • edit_plate_type to edit a plate type.

    • delete_plate_type to delete a plate type.

    Important

    You can’t edit or delete a plate type if it is used in an experiment.

  3. On the top right bar, click Save save.

Selecting a plate type opens the Plate Drops section on the right side. The section lets you enable or disable the drop(s) in the plate type. Disabling a drop means excluding it from any experiments created using the selected plate.

Plate Drops Section

Plate Drops Section