Virtual Crystal Harvesting

Virtual crystal harvesting is harvesting your crystals and defining their positions using crystal masks from the Harvesting panel. If required, you can assign cryoprotectants and soaking ingredients to your crystals.

Crystal Masks

Crystal Masks

The Harvesting panel is accessible from the Drop menu on the experiment page and has three tabs: Crystal, Cryoprotectant, and Soaking. Opening the Harvesting panel will open the Crystal tab by default.

Harvesting Panel

Harvesting Panel

Crystal Tab

Crystal tab is where you draw a crystal mask to define the crystal position. A crystal cart will be added once you draw a crystal mask.

Crystal Tab

Crystal Tab

Number

Section/Checkbox/Button Name

Description

number1

Trip Selection

Displays the synchrotron trip chips you assign your crystals to and the Unassigned Crystal chip.

Available by default, the Unassigned Crystals chip consists of a crystal(s) not assigned to a synchrotron trip.

number2

Action Menu with buttons and checkbox allowing you to:

Select All Checkbox

Select all crystal carts.

Harvest Crystal image8

Draw a crystal mask. This button is only available in the Unassigned Crystals chip.

Once you draw a crystal mask, a crystal cart will appear. The color indicator of each crystal cart (e.g. image16) is the crystal mask color (see the Crystal Masks image above).

Lock image10

Lock a crystal mask. The button will be active if you select a crystal cart.

Copy copy

Copy a cryoprotectant or a soaking ingredient.

Paste Cryoprotectant paste_cryo

Paste a cryoprotectant to the Cryoprotectant field in a crystal cart.

Paste Soaking paste_soaking

Paste a soaking ingredient to the Soaking field in a crystal cart.

Delete delete_crystal

Delete a crystal cart.

Assign Crystal assign_crystal

Assign a crystal to a synchrotron trip.

Create Synchrotron Trip create_sync_trip

Create a new synchrotron trip.

number3

Crystal Cart

Displays the crystal name, cryoprotectants and soaking ingredients you assign, and a note you add.

Cryoprotectant Tab

In this tab, you can add and modify cryoprotectants.

Cryoprotectant Tab

Cryoprotectant Tab

Each button and field in the tab enables you to:

Number

Button/Field

Description

number1

Add Well Ingredient add_well_ingredient

Use the crystal’s well ingredient(s) as a cryoprotectant.

Edit edit

Modify a cryoprotectant.

Add add

Add a cryoprotectant.

Delete delete

Delete a cryoprotectant from ROCK MAKER.

Discard discard

Cancel changes. This button will be active when you click the Add button.

Save save

Save changes.

number2

Cryoprotectant Table

Ingredient

Type the ingredient name.

Conc.

Type the ingredient concentration.

Unit

Select the ingredient concentration unit.

pH

Type the ingredient pH.

Protocol

Enter the protocol (personal note).

Soaking Tab

In this tab, you can add and modify soaking ingredients.

Soaking Tab

Soaking Tab

Each button and field in the tab allows you to:

Number

Button/Field

Description

number1

Edit edit

Modify a soaking ingredient.

Add add

Add a soaking ingredient.

Delete delete

Delete a soaking ingredient from ROCK MAKER.

Discard discard

Cancel changes. This button will be active when you click the Add button.

Save save

Save changes.

number2

Soaking Table

Ingredient

Type the ingredient name.

Conc.

Type the ingredient concentration.

Unit

Select the ingredient concentration unit.

Time

Type the soaking duration.

Unit.

Select the duration unit.

Text Info

Add a note.

To harvest your crystals virtually, go to Harvesting Crystals Virtually.