Canvas Menu¶

Located on the experiment page, Canvas is the main workspace in ROCK MAKER. It displays experiments and screens and allows you to design your experiment.

Experiment Page

There are two submenus in Canvas: Design and Image

The Design Submenu¶

The Design submenu is where you can define experiment plates by creating layers and specifying layer parameters on the Layers Design panel. It is the default submenu when you click Canvas of a new or unimaged experiment.

Design Submenu

Top Bar Buttons in the Design Submenu¶

Button	Icon	Description
Combo Action		Allows you to cut , copy , paste , refresh , undo , and redo settings.
Add Layer		Enables you to add Grid Layer, Random Layer, Custom Layer, Protein Layer, Seeding Layer, or Screen Layer.
Edit		Enables you to edit the design of a dispensed experiment.
Barcode		Enables you to print the barcode of an experiment.
Optimize		Allows you to optimize existing experiments and screens with single or multiple drop(s).
Reports		Allows you to generate a report from the available options.
More		Allows you to select Rotate Canvas, Convert to Screen, Import XML Screen, Import Fluidigm Screen, Import Text File, Export XML Screen, or Export Selected Well (TXT).
Layers		Enables you to modify the layer settings, such as naming the layer, selecting the target, and other related settings. To find out more, go to Layers Overview.
Find Drop		Allows you to highlight wells of interest using different search criteria, such as ingredient, well number, or label.
Info		Enables you to access information about a plate in detail.
Save		Allows you to save the experiment you create.
Discard		Enables you to discard experiments and changes.

Bottom Bar Buttons in the Design Submenu¶

Button	Icon	Description
Best Fit		Allows you to adjust the display of your experiment page to fit your screen.
Zoom Out		Allows you to zoom out your software window.
Zoom In		Allows you to zoom in your software window.
Plate		Allows you to go from one plate to another in an experiment. You can click the left and right arrows or the Plate button to select a plate.
Well		Allows you to go from one well to another in an experiment. If you have multiple plates in an experiment, the Well button displays the corresponding wells of a selected plate.
Drop		Allows you to go from one drop to another in a selected well.

The Image Submenu¶

Using the Image submenu, you can manage and organize your images in ROCK MAKER. It is the default submenu when you click Canvas of an imaged experiment.

Image Submenu

Top Bar Buttons in the Image Submenu¶

Button	Icon	Description
Combo Action		Allows you to cut , copy , paste , refresh , undo , and redo settings.
Switch View		Enables you to switch from Image view to Score view . Image view displays both scores and images. Score view displays scores only.
Save		Allows you to save images to your local drive.
Optimize		Lets you optimize existing experiments and screens with single or multiple drop(s).
Seed		Allows you to create a seed.
Reports		Allows you to generate a report from the available options.
More		Allows you to select Disable/Enable Imaging, Manage Imaging, Add Images, Create New Inspection, Delete Inspection, Export POI, Export XML Screen, or Export Selected Well (TXT).
Navigator		Allows you to navigate through your images using Inspections, Settings, Region, or Drop options. The Navigator panel is available on the right side of your ROCK MAKER window on click. Read more about Navigator Button. Navigator Panel
Find Drop		Allows you to highlight wells of interest using different search criteria, such as ingredient, well number, or label.
Image Now		This feature will be available soon.
Tuning		Enables you to adjust the brightness and contrast of your images.
Score		Allows you to score images and adjust the scoring settings.
Info		Enables you to access detailed information about your experiment, including experiment ID, ingredient information, imaging settings, and other related information.

Bottom Bar Buttons in the Image Submenu¶

Button	Icon	Description
Best Fit		Allows you to adjust the display of your experiment page to fit your screen.
Zoom Out		Allows you to zoom out your software window.
Zoom In		Allows you to zoom in your software window.
Plate		Allows you to go from one plate to another in an experiment. You can click the left and right arrows or the Plate button to select a plate.
Well		Allows you to go from one well to another in an experiment. If you have multiple plates in an experiment, the Well button displays the corresponding wells of a selected plate.
Drop		Allows you to go from one drop to another in a well.
Region		Allows you to go from one region to another in a drop.
Imaging Settings		Allows you to go to any available imaging settings.
Inspections		Allows you to go to any available inspections.

The Canvas menu is a visual representation of your experimental plate. For example, a 96-well experimental plate will be displayed in the Image submenu as 96 wells along with their respective drops.

The following design view shows a graphical representation of the ingredients you add to your experiment.

Ingredient Graphical Representation in Well(s) and Drop(s)

Each well on the Canvas contains the following graphical components:

Drop area. Rectangular boxes at the top of a well represent drops. In each drop that contains ingredients, relative volumes of the well and protein drops are represented by dark and light green shading. Orange represents an additive drop.

Note

Screens have no drop area since they don’t use proteins.
Well area. Below the drop area is a large square box displaying well ingredients. The thickness of the shading indicates the relative volume of each ingredient. Each ingredient type has its own associated color.
pH indicator. The pH indicator is a long rectangle at the bottom of a well. If a well contains a buffer, the pH indicator will indicate the relative amounts of the low pH and high pH stocks mixed to obtain the pH in a well. Gray shaded pH indicator indicates no stocks mixed.

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