Creating a Seed

Prerequisite:

• You have an experiment that yields crystals.

To create a seed:

1. Open an experiment.

2. Select the well(s) from which you want to create a seed. On the left panel of the experiment window, go to Drop.

   

   Drop Menu

3. On the toolbar, click the Seed button.

   

   Seed Button

4. The Create Seed dialog box will appear.

   

   Create Seed Dialog Box

   Then, you can do the following:

   Seed Source Section

   

   Seed Source Section

   Note

   Only the Seed Name field is editable.

   • Change the seed’s name or use the default name in the Seed Name field.

   Drop Image Section

   

   Drop Image Section

   • View the drop image in detail.

   Protein Formulation Section

   

   Protein Formulation Section

   • View the protein within the seed(s) in the Protein Name field. This field is uneditable.

   Conditions Section

   

   Conditions Section

   • Select the well to create a seed from the Well Number dropdown. The table below the dropdown displays information on the selected well. The information table is uneditable.

   Stabilizing Solution Ingredients Section

   

   Stabilizing Solution Ingredients Section

   • Click on the Use Well Solution checkbox to use the well solution for stabilizing solution ingredients.

   Note

   If you have more than one source well, select the source well solution from the dropdown menu next to the Use Well Solution checkbox. If you do not select any, ROCK MAKER will automatically load the first source well’s solution from which the crystal is taken.

   OR

   • Create your own ingredients by clicking the button at the bottom right corner of the Stabilizing Solution Ingredients section. Then, enter the ingredient type, name, concentration, and concentration unit in the Type, Name, Conc., and Conc. Unit fields.

   Notes Section

   

   Notes Section

   • OPTIONAL: Type any notes on your seed in the box.

5. Click the Save button on the bottom bar of the dialog box.

Creating a Seeding Layer

To create a seeding layer:

1. Open an experiment OR create a new one.

2. On the experiment page, go to the Canvas menu. Then, click Add Layer on the top bar and select Seeding Layer.

   

   Selecting Seeding Layer

3. Select a seeding layer area accordingly:

   1. To select all wells, click any well of the canvas.

   2. To select some wells, click and hold the left button of your mouse while dragging it over some desired wells.

4. The Layers Design panel will appear on the right side of your ROCK MAKER window. Then, select a seed.

   

   Choosing a Seed

5. Adjust the seed settings in the panel appearing after selecting a seed.

   

   Adjusting Seed Settings

   • Click the Name field to change the seed.

   • Select a seed volume variation from the Vary Along field.

   • Type a start seed volume in the Start field.

   Then, click Apply.

6. To modify a seed setting, click the Edit or Delete buttons in each seed box in the Seed tab.

   

   Seed Tab

7. Go to the Layers tab in the Layers Design panel (next to the Seed tab) and do the following:

   • Type a name in the Layer Name field or use the default name.

   • Select a target from the Target dropdown menu.

   • If necessary, lock the layer by clicking the Locked checkbox.

   

   Completing Layers Tab

8. Click Save

   

   Save Button

Related Topics:

• Creating an Experiment

• Seeding Layer

• Creating a Seeding Layer