Optimizing Experiments¶
ROCK MAKER makes it simple to optimize experiments and screens. Experiments can be optimized in a customized manner using the optimization experiments tool or based on the scoring of your plate using the ISO.
Optimizing Experiments as a Grid, Random, or Custom Layer¶
An optimization experiment allows you to select wells of your interest and vary their ingredients in a layer type (grid, random, or custom). This is an extremely useful feature that lets you play with the ingredients of interest in multiple combinations.
For more information on how to create an optimization experiment, go to Creating an Optimization Experiment.
Iterative Screen Optimization (ISO)¶
ROCK MAKER allows you to automatically optimize a plate based on its prior scoring. ISO can be performed in multiple rounds, with each new round optimizing the results from the previous round. During each round of optimization, the precipitant concentrations in your wells are increased or decreased by the ISO percentage you set in the General submenu of the Setup menu. You can use the default ROCK MAKER ISO percentage values or modify them accordingly. These values can also be changed for each ISO round from the Customize ISO% Values section without changing the one in the Setup menu.
You can configure the step size for each score in the ISO% column in the Well Score Configuration section of the General submenu in the Setup menu. For more information, read Creating an Iterative Screen Optimization (ISO) Experiment.
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