Random Layer

Random layer helps you design an experiment with several ingredient options. It randomly adjusts the weight, probability, pH, and concentration of selected ingredients in each well and its drop(s).

Random Layer Example

Random Layer Example

Item

Description

List of Layers

Displays all created layers. Use the right and left arrows to navigate.

Layer Options Tab
Allows you to manage:

  • Layer Name: to name the layer.

  • Target: to select a well or drop.

  • Volume: to type your desired volume.

  • Grid Steps: to define the grid steps. The default value is 10.

  • Advanced Options: to work with advanced settings, such as:

    • Layer Options: to select Override, Locked, or both.

    • Concentration: to select Volume Based Addition or Concentration Based Addition.

  • Exclude List Item: to exclude ingredient(s). Find out more about Excluding Ingredients.

Ingredients Tab

Allows you to select ingredient(s). Find out more about Creating a Random Layer.

In a random layer, the ingredients in the Group panel are classified based on their types. Please see the image below.

Group Panel

Group Panel

Ingredient Distribution

ROCK MAKER uses the assigned probability in the Layers tab of the Layers Design panel to determine which ingredient groups to distribute to a well.

Groups with higher probabilities are more likely to be used in a well. In each selected group, ROCK MAKER chooses one or more ingredients to dispense in the well based on the weight value assigned to the ingredient in the Edit Ingredient panel.

Edit Ingredient Panel

Edit Ingredient Panel

Item

Description

Name

Displays the name of the selected ingredient.

Low pH

Allows you to select a stock with low pH.

Note

This field will appear if you select Buffer in the Type field.

High pH

This field is optional. It allows you to select a stock with high pH based on the low pH you select

Note

This field will appear if you select Buffer in the Type field.

Type

Displays the ingredient type.

Concentration

Allows you to define the start and stop concentration by typing the value in the Start and Stop fields.

pH

Displays the starting and stopping pH (see the Start and Stop fields) based on the selected Low pH and High pH.

Note

If you do not define the High pH, the Stop field will be N/A.

Weight

Allows you to define the weight of the ingredient you select.

Concentration and pH Distribution

When designing a random layer, you need to define minimum and maximum values for the:

  • Concentration of each ingredient.

  • pH of each buffer ingredient.

ROCK MAKER uses the minimum and maximum values and grid steps number you can define in the Layers tab of the Layers Design panel to get sets of values to randomly design concentrations and pH levels in the random layer. The number of values is the number of steps you type in.

Each set is generated by defining numbers between the minimum and maximum values at a fixed increment determined by the number of grid steps. It results in a set of numbers for each attribute. The number of values in each set is equal to the number of grid steps.

Ingredient Variation

ROCK MAKER varies the ingredients by:

  1. Determining which ingredient groups to use in each well based on the probability you assign to each group in the Group panel.

  2. Randomly choosing one or more ingredients from each selected ingredient group based on the ingredient group’s probabilities.

  3. Distributing the selected ingredients into the well.

Excluding Ingredients

Exclude List Item allows you to choose pairs of ingredients not to be mixed in random layer wells. As a result, none of the combinations of the Exclude List Item occurs.

Exclude List Item

Exclude list Item

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