Imaging Settings

Imaging Settings are a collection of settings that tells ROCK IMAGER how an experiment plate should be imaged. These are defined in the Imaging Settings dialog box, available from the Imaging Settings node under the Imaging node on the Explorer.

The Imaging Settings dialog box is divided into left and right areas. The fields at the right will change based on the Imager you select at the left.

Any changes you make here will override the imaging settings stored in the ROCK IMAGER software.

Imaging Settings are defined at three levels. The uppermost or global level is through the Imaging folder on the Explorer where imaging settings are associated with global imaging schedules.

When you create a project, you select one of these global imaging schedules on the Project screen. This becomes the default imaging schedule for any experiments created under that project.

When an experiment is created, it defaults to the imaging schedule used by its parent project and ultimately uses the imaging settings associated with that imaging schedule. Both imaging schedule and imaging settings are customizable at the experiment level.

Once imaging is done, you can even override these experiment-level imaging settings for each drop and region of interest. The purpose of this is to allow you to find the optimal settings for imaging.

For example, you may find that the imaging settings assigned at the experiment level may not provide enough contrast in one drop. In such a case, you could create three different imaging settings with varying polarizer and gamma settings and assign them to the drop in question. This would result in three separate images of the drop.

After the next imaging session, you would select the drop image on the Canvas and select Imaging Settings after selecting Navigate by on the Navigator. Those three images would appear on the Navigator. Then you could compare those images in detail on the Compare tab by pressing the Ctrl key and selecting all of them on the Navigator.

Related Topics