Protein Layers

A protein layer specifies the protein formulation that will be used in one of the drops in each well of the layer. Each protein layer applies only to one drop per well. When working with plates that allow for multiple drops, you will need to create a protein layer for each drop.

Protein layers can span multiple other layers. For example, if you design an experiment that contains a screen layer, a random layer, and a grid layer, you can use the same protein formulation in all three layers by drawing a protein layer that encompasses all of them.

On the canvas, a protein layer is represented graphically by shading in one of the drops located above each well, as illustrated in the following example.

Protein Layer

Varying Well and Protein Volumes

ROCK MAKER allows you to define how the volumes of the protein formulation and well solution that make up the drop will vary across the layer.

Varying Well Solution Volume

1. Under Well Solution Volume, select an option from the Vary list.

2. Type the starting volume into the Start box.

3. Type the ending volume into the Stop box.

Varying the Protein Volume

1. Under Protein Volume, select an option from the Vary list.

2. Type the starting volume into the Start box.

3. Type the ending volume into the Stop box.

Related Topics

• Layers Overview

• Screen Layers

• Grid Layers

• Random Layers

• Custom Layers

• Fragment Layers