Creating a Fragment Layer

When designing protein crystallization experiments in ROCK MAKER, you can add multiple fragments to a desired experiment. Creating a fragment layer involves specifying target well drops and selecting fragment screens.

Prerequisites:

• You have created a Fragment Screen.

• You are running ROCK MAKER version 3.16 or newer.

To create a fragment layer:

1. In the Explorer, expand the Project node, open the desired experiment and then go to the Canvas tab.

2. Choose from the following options:

   • Click the Fragment button in the Add Ingredients group on the Design tab. Then, drag your mouse to the canvas to select the desired well(s) for your fragments. On the Select Screen Fragment panel, select the desired fragment screen from the list.

     

     Selecting the Desired Well(s) to Which You Will Add Fragments

     OR

   • Drag the desired fragment screen from the Explorer to the desired drop location(s) on the Canvas tab.

     

     Dragging a Fragment Screen from the Explorer to the Canvas

3. In the Edit Fragment Screen Layer panel, type a descriptive name for the layer in the Name box. Then, select the desired target drop, either drop 1 or 2, in the Target box and click Update.

   

   Selecting the Desired Fragment Screen to Add to the Protein Drops

4. ROCK MAKER will automatically assign the fragments to all the wells that you have previously selected.

   

   The Fragment Layer in Drop 1 is Represented by a Dark Shading

5. To add another fragment layer to your drops, repeat steps 1 - 2.

6. Click the Save button or press Ctrl+S on your keyboard to save the changes.

   Note

   If you haven’t added a protein layer to the drops to which you have added a fragment layer, please proceed to add a protein layer.

Related Topics

• Fragment Layers

• Adding Ingredients and Working with Layers

• Creating a Protein Layer

• Creating a Fragment Screen